Lc quantitative analysis using lc480 v1 en 20200401 sm 4. It enables the user to monitor the amplification of the pcr product simultaneously in realtime allowing the accurate quantification of target sequences. The lightcycler 480 realtime pcr system roche applied science brings you one more breakthrough in the realtime pcr analysis of gene expression and genetic variation roche applied sciences cuttingedge gene analysis technology is now available in a highthroughput, microwell platebased cycler format 96 or 384well. Roche instruments are well known for an userfriendly interface. Mar 31, 2020 i am trying to do a high resolution melting thermal shift assay by applying a thermal shift assay control kit, to a lightcycler nano instrument from roche. This lot comes with everything shown in the photos, to include. Traditional methods of diagnosis, including wet preparation, can be unreliable. Roche applied science lightcycler 480 realtime pcr system. Roche molecular biochemicals lightcycler operators manual. Providers of deep well plates, pcr plates and tubes, silicone sealing mats, reservoirs, and other laboratory plastic consumables. Rapid highthroughput, platebased realtime pcr amplification and detection instrument. The lightcycler instrument of roche molecular biochemicals, mannheim, ge rmany, is a thermocycler for the rapid analysis of pcr applications. Ukbased itis will manufacture the new instrument for roche, which said that it will complete the firms portfolio for lowthroughput realtime pcr systems. The lightcyclers small reaction vessels allow for efficient temperature ramping, with cycling rates of less than 20 secondscycle.
With realtime pcr and especially the roche applied science lightcycler systems, minimal amounts of dna can be replicated very rapidly and detected easily. Lightcycler taqman master is a readytouse reaction mix designed for the hydrolysis probe detection format in lightcycler capillaries using the lightcycler carouselbased system. Introduction the accuchek performa nano system the accuchek performa nano meter is designed to be used with the accuchek performa and accuchek inform ii test strips to quantitatively measure glucose in fresh venous, arterial, neonatal, and capillary whole blood as an aid in monitoring the e ectiveness of glucose control. The lightcycler carouselbased system lightcycler 1. This is a midprice segment of the market qpcr machine dedicated for life science research only. Based on a comprehensive improvement of the peltierbased technology incorporated in a silver block, the instrument provides extraordinary welltowell temperature homogeneity and maximized interwell, intercycle reproducibility. Trichomonas vaginalis is the agent of a highly prevalent sexually transmitted infection sti that can result in vaginitis, urethritis, and preterm birth. The kit contains a novel dna polymeraseengineered via a process of molecular evolutionresulting in a unique enzyme specifically designed for realtime quantitative pcr qpcr. Roche today announced the global launch of new software updates, including advanced high resolution melting analysis and qualitative gene detection modules for the light cycler 96 and new support features for the lightcycler nano system. Next to its fancy design it is also one of the smallest realtime pcr systems available. Lightcycler nano system performance data roche life science.
Roche lightcycler ii laboratory thermal pcr system. The lightcycler nano can perform duplex assays on up to 32 samples. In this study, we describe the adaptation of an existing pcr method for specific detection of t. Lightcycler technology is the most innovative and rapid possibility for carrying out and simultaneously evaluating pcr experiments. Find product specific information including cas, msds, protocols and references. The faststart essential dna green master is designed for research studies. May 17, 20 the lightcycler nano system features a plugandplay device with stylish design, making it the smallest and most silent realtime pcr system on the market. Roche accuchek performa nano user manual pdf download. The roche lightcycler 480 rtpcr system combines amazing speed with exceptional accuracy and will meet the needs of a broad range of scientific applications in genomics research including array validation, geneknockdown studies, and snp. C automated peak identification multiplexing mygo pro nano precalibrated dyes 23 9 7 2 user generated dye files warranty mygo pro nano extended warranty available genotyping mygo pro nano automated hrm automated taqman genotyping enhanced background subtraction multiplex level. Roche diagnostics introduces ultracompact lightcycler nano. The roche lightcycler 480 system another breakthrough in realtime pcr analysis of gene expression and melting curvebased mutation analysis. Kapa sybr fast qpcr master mix 2x kit sigmaaldrich.
The system features the lightcycler 480 instrument, a versatile, platebased realtime pcr device that supports mono or multicolor applications, as well as multiplex protocols. Lc quantitative analysis using lc480 v1 en 20200401 sm 41. The benchtop instrument is easily customizable to meet changing user requirements, and can be integrated into everyday use as a robotically controlled, automated high. For todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected from a lightcycler system. In combination with the lightcycler nano or lightcycler 96 system and suitable pcr primers, this kit allows very sensitive detection and. However, roche diagnostics gmbh reserves the right to make any changes necessary without notice as part of ongoing product development. In addition, genespecific thresholds can be defined manually to change the positivenegative call. The lightcycler nano software runs on pcs and macs. Roche lightcycler realtime pcr systems system evolution meets intelligent design. Preparation of the lightcycler faststart dna master 14 6.
Ever since its introduction in 1998, the roche applied science lightcycler system has stood for outstanding performance and maximum flexibility. Roche lightcycler nano was relased in november 2012 at american society for human genetics annual meeting in san fransisco. Lightcycler 480 quick guide page i 51250011 general information the contents of this document, including all graphics and photographs, are the property of roche diagnostics. Reaction vessels for lightcycler carouselbased system instruments manufacturer. The lightcycler 480 instrument ii is a rapid, platebased, thermal block cycler with integrated realtime, online detection capabilities. Roche diagnostics has launched the new lightcycler nano realtime pcr system for use in research in the us. The cycler has 4 detection channels, which detect hydrolysis 5nuclease taqman probes either as single color or multiplex samples. The instrument the lightcycler instrument figure from roche diagnostics is an extremely fast thermocycler with online fluorescence detection. Roche launches lightcycler nano realtime pcr system in. This platform comes with a redesign software that is designed to provide guided navigation. Lightcycler nano software highlights roche life science. Roche lightcycler 96 biotecon diagnostics biotecon. Roche inks lightcycler nano oem deal with itis genomeweb.
Oligo probe 1 is labeled at the 3 end with a donor reporter and oligo probe 2 is labeled at its 5 end with one of a few available acceptor reporters. Roche launches new software updates for its lightcycler 96 and lightcycler nano pcr systems. High resolution for copy number determination and melting curve. With a total of 6 detection channels, this compact cycler offers the highest flexibility and allows single color and multiplex testing.
A lightcycler probe system consists of a pair of singlestranded fluorescentlabeled oligonucleotides. Service manual light cycler please note that this is only. The faststart essential dna probes master is a readytouse hot start reaction mix designed. The realtime pcr assay design was based on fret probe chemistry. Faststart essential dna green master roche online technical. Intuitive in design and capable of being used with or. When used with the lightcycler nano or lightcycler 96 system, this kit is ideally suited for hot start pcr applications. Roche diagnostics introduces ultracompact lightcycler nano realtime pcr system in us small platform supporting stateoftheart technology for fast pcr protocols introduced at american society of. The lightcycler is available in 96, 480 and 1536 sample configurations each of which can perform 4 dye multiplexing.
The lightcycler nano instrument looks great on your bench and produces beautiful data. The lightcycler 480 system is a highperformance, medium to highthroughput pcr platform 96 or 384well plates that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. Quickly and easily use demo files and predefined default settings for each application, the lightcycler nano software has predefined templates and temperature profile building blocks e. Lightcycler 480 instrument ii roche applied science. Roche lightcycler whether your interest is in gene expression profiling or in detecting genetic variations, there is a member of the lightcycler system family offering the analytical performance and throughput you need for your research. The lightcycler nano, lightcycler 96, and lightcycler 1536 softwares provide an automatic cq calling based on a fixed threshold set by the system. It contains faststart taq dna polymerase for hot start pcr, which significantly improves the specificity and sensitivity of pcr by minimizing the formation of nonspecific amplification products.
New york genomeweb news roche applied science today said that it has inked an oem agreement with itis life sciences for distribution of the new lightcycler nano instrument. Use of the roche lightcycler instrument in a realtime pcr. Ii contact addresses manufacturer roche diagnostics ltd. Innovative laboratory products, llc pcr plates roche. As the cq values are calculated once for each sample. The lightcycler nano instrument is an ultracompact realtime pcr system, supporting stateoftheart technology for fast pcr protocols. Compact and easy to use, the lightcycler 96 system primary is the ideal companion for applications including gene expression analysis, gene detection, mutation detection, methylation analysis, mirna research, and relative quantification of target genes. The software is compatible with the regulations for electronic records subpart b. Realtime pcr master mixes and instrument compatibility. The system features the lightcycler 480 instrument, a versatile, platebased realtime pcr device that supports mono or multicolor applications. An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is accessible to any user. The combination of both components allows complex applications such as product analysis, quantification and mutation analysis. Like all roche realtime pcr systems, the lightcycler nano system uses excellent thermal control for high reproducibility of results obtained with the 32 samples tested in the same run.